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0.05.Conclusion Using XT-2000i Automated Hematology Analyzer to test the reticulocyte the reproducibility is better,and the linearity can content well with clinic,the rate of carry contamination is low,the stability is better too.Compared XT-2000i with the manual methods,the relativity is better. It can replace the manual methods.
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OBJECTIVE To explore the positive rate of intercellular adhesion (ica) operon and its production- polysaccharide intercellular adhesion(PIA) and the relationship between the rate and adhesion of Staphylococcus epidermidis. METHODS Amplifying icaA of ica operon by PCR and then detecting it by agarose electrophoresis, detecting PIA by Congo red agar (CRA) to detect its adhesive ability by infusion set. RESULTS The icaA positive rate of S.epidermidis was 51.5%; the PIA positive rate was 50%; there was significant difference between the clone numbers of PIA positives and negatives (P
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Objective;Discussing clinical utilization of Automation Urine Analyszer in the testing Hydrothorax and Hydroperitonium. Methods:Testing 80 specimen got from our clinci and patient by using Uritest-200 equipemnt to get the results of PRO and SC, using UF - 100 to get the results of RBC,WBC,EC which can be compared with the results which got by handwork. Results:Comparison between Uritest-200 and handwork method, PRO and SG usex2 examination, the results have very siginficant differ( P
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<p><b>OBJECTIVE</b>To determine the relationship between vitamin D receptor (VDR) gene polymorphism and osteoporosis in postmenopausal women.</p><p><b>METHODS</b>The polymerase chain reaction-restriction fragment length polymorphism was used to detect VDR genotype in 40 patients with osteoporosis and 21 healthy postmenopausal women.</p><p><b>RESULTS</b>In the patients with osteoporosis, the bb, Bb, and BB genotype accounted for 82.5%, 17.5% and 0, respectively; in healthy groups, they were 85.71%, 14.29% and 0, respectively (P>0.05).</p><p><b>CONCLUSION</b>Significant association between VDR genotype and osteoporosis in Chinese women was observed in this study.</p>
Subject(s)
Female , Humans , Middle Aged , DNA , Genetics , Metabolism , Deoxyribonucleases, Type II Site-Specific , Metabolism , Gene Frequency , Genotype , Osteoporosis, Postmenopausal , Genetics , Polymorphism, Genetic , Receptors, Calcitriol , GeneticsABSTRACT
OBJECTIVE:To determine the resistance of clinically isolated bacteria to meropenem METHODS:The resistance of 150 clinically isolated strains to meropenem and several other antibiotics was detected by K-B method and the drug resistant spectra to E coli were compared between meropenem and imipenem RESULTS:The sensitivity of 130 clinical isolates to meropenem was 100% No cross resistant strains of E coli was found to meropenem and imipenem CONCLUSION:Meropenem is highly sensitive to the clinical isolates,and its antibacterial spectrum is similar to that of imipenem
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Objective:To study the resistance and its tendency of E.coli to antibiotics was to serve the rational administration in clinic.Methods:WHONET5 was employed to analyze the drug sensitivities of 616 E.coli isolated from the clinical specimens from January,2004 to July,2006 in hospital.Results:The resistance rates of E.coli to Ampicillin,Piperacillin,Amoxicillin/cla,Trimethoprim/Sulfamethoxazole and Ciprofloxacin exceeded 60%,and they were about 50% to Gentamicin,Cefazolin and Cefuroxime.In addition,the resistant rates were20% infra to Piperacillin/tazobactam,Cefepime,Ceftazidime,Cefoxitin,Amikacin and Furantoin,and there was no resistance to Imipenem.The detection rates of ESBLs to E.coli was 27.96%,32.68%,35.12% in 2004,2005,2006 respectively.Conclusions:The resistance rates of E.coli to Penicillins,Piperacillins/Clavulanic acid,Sulfanilamide,Quinolone,Gentamicin,the first and second generations Cephalosporins were high,and they were not suitable for clinical use.To Piperacillins/tazobactam,fourth generation Cephalosporins,Cephamycins,Imipenem,Amikacin and Furantoin,the resistance rates were relatively low,and they could be used clinically.The third generation Cephalosporins should be used cautiously due to its feasibility of inducing ESBLs even though its resistant rates were low.The production rate of ESBLs of E.coli ascended year by year.